The amount of plasmid for ligation reaction
Posted 17 February 2005 - 08:21 AM
Posted 17 February 2005 - 01:08 PM
I suggest you do some controls by transfecting GFP and try different conditions (µg of plasmid, µl of transfectant).
Posted 19 February 2005 - 03:06 AM
i'm still working with the very old lipofectamine reagent (yes it still exists ) and on the reference guide they give a graphical analysis on the efficiency of transfection. You can go to the page at :
(it's not the one from invitrogen because they've upgraded the notice and didn't keep the table).
But Simonsays is right. You have to adapt conditions for your experiments...