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Smearing after immunoprecipitation


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#1 hofsethl

hofsethl

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Posted 17 February 2005 - 07:41 AM

To anyone who can help... We have been using homogenized mouse tissues and have immunoprecipitated pRb (the retinoblastoma protein) with a monoclonal antibody (linked to agarose), then immunoblotted with a polyclonal to E2F1. Although we get a band at the molecular weight of E2F1, the bands are heavily 'smeared'... both above and below the center of the band.

Has anyone experienced this?... and, can anyone give advise to what this phenomenon might be?

Thank you very much in advance...

Edited by hofsethl, 17 February 2005 - 08:10 AM.





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