Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo

transfection of Caco2 cells


  • Please log in to reply
1 reply to this topic

#1 sophie

sophie

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 16 February 2005 - 06:27 AM

I haven't been very succesfull in transfecting Caco2 cells so far. Does anybody have experience with it and could tell me which conditions work out the best like confluency, and transfections agents to use (lipofectamine 2000, oligofectamine ...)? Thanks.

#2 BryanW

BryanW

    member

  • Active Members
  • Pip
  • 17 posts
0
Neutral

Posted 16 February 2005 - 11:13 AM

The most effective liposome-based transfection reagents are GenCarrier, Lipofectamine2000(CD) followed by fugene-6 and genePORTER. In our hands, GenCarrier tops the list in low cytotoxicity, high transfection efficiency, much lower price and broad cell types - primary or established, adherant or suspension, serum or no serum.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.