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[sophie]

I haven't been very succesfull in transfecting Caco2 cells so far. Does anybody have experience with it and could tell me which conditions work out the best like confluency, and transfections agents to use (lipofectamine 2000, oligofectamine ...)? Thanks.

[BryanW]

The most effective liposome-based transfection reagents are GenCarrier, Lipofectamine2000(CD) followed by fugene-6 and genePORTER. In our hands, GenCarrier tops the list in low cytotoxicity, high transfection efficiency, much lower price and broad cell types - primary or established, adherant or suspension, serum or no serum.