I am doing yeast two-hybrid now. I picked up a colony from my plate and grow it in appropriate medium. Before I did other exp with the yeast medium( test the bait expression) I spread 20ul yeast on appropriate SD plate(just use 1/5 of the 10cm plate) for stock. I got big smear on the plate, then I use some of the smear to replate on another apporiate 10cm plate but still got smear. I am wondering what the smear is? Is that only because I plate too much in a small area so the yeast does not have enough area to form colony or the yeast medium has been contaminate by bacteria or something else?
Yeast grows as smear instead of colonies
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