transfection of single copy of plasmid per cell
Posted 15 February 2005 - 07:10 PM
Does anyone know of a transfection procedure where mamallian cells can be transfected and will result in a single copy of the plasmid for each cell transfected?
Something like transformation of E.Coli, where it is thought that only a single copy of the plasmid transforms each cell.
To my knowledge, with the current mammalian transfection techniques many copies of plasmid are transfected into one cell. Or am I wrong?
So, if anyone can give a suggestion how can I transfect mammalian cells with only one plasmid for each cell, I would appreciate it.
Posted 15 February 2005 - 09:22 PM
If this is the case, then youll need to make sure your DNA gets into the genome. Remembering way back to college, you might try some sort of "knock-in" strategy via a double crossover using a cassette.
Just a stab in the dark, and Im probably wrong so take my reply with a grain of salt
Posted 16 February 2005 - 09:33 AM