We have trouble with the storage of the homemade taq.
We use the Bio Rex 70 Resin for its purification.
We have tried with different glicerol qualities and concentracions, autoclavated or not.
With 50% glycerol it looses activity and with 30% it gets frozen.
What can we do?.
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homemade taq
Started by biotec, Feb 15 2005 10:34 AM
2 replies to this topic
#2
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Posted 19 February 2009 - 06:41 AM
biotec, on Feb 15 2005, 10:34 AM, said:
We have trouble with the storage of the homemade taq.
We use the Bio Rex 70 Resin for its purification.
We have tried with different glicerol qualities and concentracions, autoclavated or not.
With 50% glycerol it looses activity and with 30% it gets frozen.
What can we do?.
We use the Bio Rex 70 Resin for its purification.
We have tried with different glicerol qualities and concentracions, autoclavated or not.
With 50% glycerol it looses activity and with 30% it gets frozen.
What can we do?.
Why can't you just buy them .. it's not that expensive..
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#3
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Posted 20 February 2009 - 12:09 AM
dialysis is the best in my experience.
I concentrate the lysate with vivaspin-centrifuge, then put it in a dialysis tube for 12hours in the storage buffer (50mM Tris-HCl pH 7.9, 50mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mM PMSF, 50% glycerol )
I concentrate the lysate with vivaspin-centrifuge, then put it in a dialysis tube for 12hours in the storage buffer (50mM Tris-HCl pH 7.9, 50mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mM PMSF, 50% glycerol )
