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realtime RT-PCR/internal control


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#1 molcyt

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Posted 14 February 2005 - 09:38 AM

Hi,

I will use real time RT-PCR to detect gene expression. But I could not decide which internal control shall I use. Do you have any advise about that? And if you have a good internal control you used for your research before, could you please give me the primer sequence. Thanks a lot

#2 bob1

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Posted 14 February 2005 - 03:31 PM

Hi

There are a range of internal controls possible, most people use beta-Actin, 16s RNA or GAPDH. There should be primers avaliable for these from most primer supply companies.

good luck
bob

#3 labmestake

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Posted 14 February 2005 - 05:38 PM

also consider RNA pol II

#4 jadefalcon

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Posted 15 February 2005 - 05:59 AM

a paper you might be interested in :

Validation of housekeeping genes for normalizing RNA expression in real-time PCR
Keertan Dheda, Jim F. Huggett, Stephen A. Bustin, Margaret A. Johnson, Graham Rook, and Alimuddin Zumla
BioTechniques Vol. 37, No. 1: pp 112-119 (Jul 2004)


mike
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