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[patirl]

hi all

could anyone tell me what the best loading control is for western blotting. I work on yeast and so I'm wondering if people use actin or is there something very specific to yeast people.

thanks

Pat

[patirl]

just thought that I would answer my own question

Actin is not cell cycle regulated and therefore a good loading control for cell cycle work. others including phospho-glycerate kinase and GAPDH do show some differences during the cell cycle