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Bradford Protein Assay


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#1 anonymous

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Posted 09 February 2001 - 10:00 PM

The recommended wavelength for the Bradford Protein Assay is 595nm. I don't have accessto a microtitre plate reader with this filter at the moment. BioRad state that you can use a range of wavelengths between 465 and 595nm. Has anyone tried a wavelength other than 595nm?

Thanks in advance for any help.


#2 anonymous

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Posted 13 February 2001 - 10:00 PM

My reader only allows me to read at 620!The proteins I have isolated seem to work.

#3 anonymous

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Posted 23 February 2001 - 10:00 PM

You can make a wavelength scanning to see which wavelength you can use. The Biorad must have established their recommendation by this way.




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