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SF9 cells


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2 replies to this topic

#1 promilasharma

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Posted 13 February 2005 - 09:11 PM

Does anyone have any idea which antibiotic shoud we use for SF9 cells ? Moreover which protocol is better for transfection of SF9 cells using calcium chloride or lipfectin?
Thanks.

#2 seasnake

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Posted 16 March 2005 - 07:01 AM

I use pen/strep at the concentrations one would use in general cell culture. For the transfection I use cellfectin with good results. havent tried any other method yet.

#3 mnqcljsm

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Posted 16 March 2005 - 08:05 AM

i use blasticidin and cellfectin on a similar cell line




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