Hi,guys:
I am working in suspension cell now, and I want to check it on confocal microscope,How can these cells attach to coverslips?
thank you very much!
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Simonsays
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Posted 11 February 2005 - 02:37 PM
pretreat your coverslips with 0.1 mg/ml poly-L-lysine for 30 minutes, wash them with PBS and let them dry. Load your cells and let them attach (may take few hours). It worked for a friend of mine who worked with DT40 (suspension cells) and wanted to check them out in a fluorescence microscope.
Simon
Simon
