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Western Blot Loading Control


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#1 Cell

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Posted 11 February 2005 - 11:48 AM

I am looking for a loading control for a western blot that is well above or below 50 KDa. Actin is to close to my bands to be a loading control, and I am looking for my protein in skin.

Any suggestions????

#2 fred_33

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Posted 13 February 2005 - 08:16 AM

hi
you can use the well known p53 protein or the TBP protein (Tata Box Binding Protein).

#3 george@CASE

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Posted 14 February 2005 - 12:16 PM

tubulin? not really sure if that'll work.




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