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Multiplex Cytokine Assays - Antibody coupling


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2 replies to this topic

#1 lizzshead

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Posted 10 February 2005 - 06:12 AM

I am looking for advice from anyone that has bound antibody to the microspheres used in the Luminex multiplex cytokine assay. I am looking for a protocol and also general information on how well these 'home-made' microspheres work.
Thanks in advance.

#2 purduein

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Posted 08 March 2005 - 10:21 AM

Please see the following hyperlink for a protocol to couple antibodies to microspheres:

http://luminexmachin...ated=1057511885

Admittedly, I am using this protocol for the first time myself, but I was directed to it by Luminex Corporation. Hopefully it will bring both of us some success.

Good luck and let me know if I can be of further help.

#3 hugok

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Posted 27 August 2009 - 03:45 AM

any updates on coating antibodies to luminex multiplex beads?




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