Putting more than one membrane in a container...
Posted 09 February 2005 - 05:09 PM
My question is:
After SDS-PAGE I usually transfer onto PVDF membrane. Let's say I ran two gels and then transfered those gels onto 2 PVDF membranes. Can I put the two PVDF membranes in the same container and do blocking, first and second antibodies, plus washing in that container? (I am probing both membranes with the same antibodies.)
I ask this because when I do this, it seems that the membranes stick together, so I am worried that when I put the first antibody in the container (about 5ml of diluted antibody), the antibody will not reach the bottom membrane since the membrane on top is stuck to the bottom membrane. However, a colleague told me that this is no problem actually, because the antibody will seep in even though the membranes look like they're stuck together.
I hope I'm being clear here. Anyone think this is no problem?
Posted 10 February 2005 - 06:00 AM
Posted 10 February 2005 - 09:52 PM