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B-gal staining of cells


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#1 Cindy

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Posted 09 February 2005 - 03:29 PM

I'm working with suspension cells and I was wondering if there's a good way to stain for B-gal expression. I've done it before with adherent cells, but I'm not sure how to do it with adherent ones. Also, I don't have a kit to do it, and I'm unsure of the exact reagents I'd need to stain (other than that I need Xgal). Any help with a protocol or something like that would be greatly appreciated B)

#2 ytaolle

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Posted 14 March 2005 - 10:48 AM

I haven't actually stained non-adherent cells, however, I just recently read a protocol that covers that. The Xgal staining protocol is pretty far down the page.

http://www.stanford....helper_dep.html

Hannah




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