Posted 08 February 2005 - 07:32 AM
I am trying to express protien, with induction of IPTG( 1mM) for 4 hours, but the expression is low. So, can I try increasing the con. What could be highest concentration of IPTG I can use??
Posted 08 February 2005 - 09:47 AM
Instead, try to induce at higher density (OD600 > 0.6), or induce for longer time (overnight at 30 degrees). Or use a different strain. Some strains are optimized for expression of mammalian proteins.
Posted 21 February 2005 - 11:25 PM
If IPTG is aded in the beginning itself then the cells start expressing the protein immediately and most of the cellular resources are siphoned off and the cell division machinery is deprived.
National Chemical Laboratory
Posted 22 February 2005 - 11:35 AM