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gene linearization for transfection


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#1 pascal

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Posted 08 February 2005 - 03:48 AM

My viral vector (pBabe) must be linearize before transfection
- with CLONfectin?
- with electroporation
thank you
Pascal

#2 fred_33

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Posted 08 February 2005 - 05:02 AM

hi
for electroporation, and transfection, there's no need to linearize your vector. Be sure adding a selection pressure (like antibiotic) two days after transfection in order to eliminate cells that don't have integrated your vectorin their genome.




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