My viral vector (pBabe) must be linearize before transfection
- with CLONfectin?
- with electroporation
thank you
Pascal
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fred_33
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Posted 08 February 2005 - 05:02 AM
hi
for electroporation, and transfection, there's no need to linearize your vector. Be sure adding a selection pressure (like antibiotic) two days after transfection in order to eliminate cells that don't have integrated your vectorin their genome.
for electroporation, and transfection, there's no need to linearize your vector. Be sure adding a selection pressure (like antibiotic) two days after transfection in order to eliminate cells that don't have integrated your vectorin their genome.
