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Where I find hTert and Tsv40 genes


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#1 pascal

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Posted 08 February 2005 - 03:45 AM

Hello
I search 2 genes for mammalian cell immortalization. One is the antigen T gene of sv40 virus the other one is the catalytic part of the human telomerase (htert) gene.
thank for your help
Pascal

#2 jadefalcon

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Posted 08 February 2005 - 06:10 AM

are you seraching for the nucleotide-sequences of thes genes, or do you want to have the nucleic-acid itself?

the sequences should be available in public databases such as

http://www.ncbi.nlm....i?db=Nucleotide

with that knowledge, the nucleic-acids of these genes themselves should be more or less easy to amplificate by PCR out of the respective material....

mike
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#3 pascal

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Posted 08 February 2005 - 06:45 AM

I wanted the gene in plasmid/vector for eukaryotic cell transfection or electroporation.




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