Transfection with Effectene
Posted 07 February 2005 - 06:11 PM
I encounter a problem in transfecting HEK 293 A cell. The first time I transfect with Qiagen Effectene reagent and the cell is pretty good and transfection of GFP shows that it's successful. However, I did transfection again with the same reagent, but right after I transfect the cell, they float. And It does not seem to be blown up.
Plus, I am always using antibiotics and serum containing medium cause the protocol siad"can contain". And previously, it works.
Thanks a lot
Posted 14 March 2005 - 08:05 PM
Posted 15 March 2005 - 01:59 AM
for transfection, you can use serum during transfection. But 293cells do not need serum for achieving good efficiency.
But may i advice you to not use antibiotics during transfection. your cells are enough damaged by the mixture lipid molecule DNA, and do not need to deal with antibiotics too....