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quesiton about GST fusion protein


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#1 kroger

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Posted 07 February 2005 - 08:23 AM

hi,guys:
I am trying to purify GST-fusion protein, my protein is not stable and easy to degrade. Then I try to cleave the GST, but it can't be cleaved,GST keeps binding on beads.what's the problem?
thanks a lot

#2 sharath

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Posted 21 February 2005 - 11:29 PM

what method do you employ to cleave GSt
Sharath B.




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