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Storage of Midipreps - Molecular Biology - BioForum

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Storage of Midipreps


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3 replies to this topic

#1 Trinity

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Posted 07 February 2005 - 01:29 AM

Hi!

At what temperature do you store a pasmid midi prep? I always stored it at - 20 C. Someone told me its better to store it at - 4 C in the fridge!

#2 fred_33

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Posted 07 February 2005 - 02:02 AM

hi
i store my minipreps always at -20! Once, when i need often a miniprep, i stored an aliquot in the fridge. After this use i forgot it in the fridge and after two month, i check my prep on agarose gel : it was a nice smear with one band in the middle !!!

#3 ros

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Posted 07 February 2005 - 02:16 PM

Hi,

You should really store your plasmids in the fridge (4 degrees Celcius) because constant freeze-thawing can nick the DNA. However if you want long term storage, the -20 degrees is probably OK.

#4 jadefalcon

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Posted 07 February 2005 - 02:26 PM

hi!

a similar topic has been discussed here:

http://www.protocol-...self defrosting

basically, both 4 or -20C is ok, though there are some people who believe that self defrosting freezers can do very bad things to DNA :blink:

mike
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