Hi!
At what temperature do you store a pasmid midi prep? I always stored it at - 20 °C. Someone told me it´s better to store it at - 4 °C in the fridge!
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#2
fred_33
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Posted 07 February 2005 - 02:02 AM
hi
i store my minipreps always at -20°! Once, when i need often a miniprep, i stored an aliquot in the fridge. After this use i forgot it in the fridge and after two month, i check my prep on agarose gel : it was a nice smear with one band in the middle !!!
i store my minipreps always at -20°! Once, when i need often a miniprep, i stored an aliquot in the fridge. After this use i forgot it in the fridge and after two month, i check my prep on agarose gel : it was a nice smear with one band in the middle !!!
#3
ros
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Posted 07 February 2005 - 02:16 PM
Hi,
You should really store your plasmids in the fridge (4 degrees Celcius) because constant freeze-thawing can nick the DNA. However if you want long term storage, the -20 degrees is probably OK.
You should really store your plasmids in the fridge (4 degrees Celcius) because constant freeze-thawing can nick the DNA. However if you want long term storage, the -20 degrees is probably OK.
#4
jadefalcon
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Posted 07 February 2005 - 02:26 PM
hi!
a similar topic has been discussed here:
http://www.protocol-...self defrosting
basically, both 4 or -20°C is ok, though there are some people who believe that self defrosting freezers can do very bad things to DNA
mike
a similar topic has been discussed here:
http://www.protocol-...self defrosting
basically, both 4 or -20°C is ok, though there are some people who believe that self defrosting freezers can do very bad things to DNA
mike
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