Previously used Bioline kit, have tried QIAmp DNA stool mini kit but am getting really poor yield, can anyone recommend any other kits or methods?
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Kit for DNA Extraction from faeces
Started by Fiona, Feb 04 2005 06:41 AM
3 replies to this topic
#2
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Enthusiast
Posted 19 February 2009 - 09:33 AM
We have a reagent AquaPrecipi for fecal PCR inhibitor removal. Fecal DNA is ready for PCR after it is precipitated with AquaPrecipi and ethanol. Fecal DNA analysis is an excellent noninvasive method for wildfile, GI infections, GI cancers, and genotyping studies.
PCR amplification of human stool DNA.
Lane 1: PCR of DNA precipitated by isopropanol
Lane 2: PCR of DNA precipitated by AquaPrecipi/ethanol.
Lane 3: PCR of DNA precipitated by AquaPrecipi/isoproanol.
PCR amplification of human stool DNA.
Lane 1: PCR of DNA precipitated by isopropanol
Lane 2: PCR of DNA precipitated by AquaPrecipi/ethanol.
Lane 3: PCR of DNA precipitated by AquaPrecipi/isoproanol.
#3
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Spirit
Posted 19 February 2009 - 10:28 AM
Fiona, on Feb 4 2005, 09:41 AM, said:
Previously used Bioline kit, have tried QIAmp DNA stool mini kit but am getting really poor yield, can anyone recommend any other kits or methods?
I have just recently ordered a sample kit from Zymo Research (with 5 reactions) and it worked fine for me. The sample kit is for free.
http://www.zymoresearch.com/products/dna/z...cal_dna_kit.asp
Cheers!
"You can give somebody a book on 'How to ride bike' and then test that person on that knowledge. Even if that person gets an "A", it doesn't mean that he or she can ride a bike."
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---this is what I am telling myself when I get a bad grade....as long as you don't loose your passion, you'll be fine.....V
#4
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Enthusiast
Posted 16 November 2009 - 01:23 AM
AquaPlasmid, on Feb 19 2009, 09:33 AM, said:
We have a reagent AquaPrecipi for fecal PCR inhibitor removal. Fecal DNA is ready for PCR after it is precipitated with AquaPrecipi and ethanol. Fecal DNA analysis is an excellent noninvasive method for wildfile, GI infections, GI cancers, and genotyping studies.
PCR amplification of human stool DNA.
Lane 1: PCR of DNA precipitated by isopropanol
Lane 2: PCR of DNA precipitated by AquaPrecipi/ethanol.
Lane 3: PCR of DNA precipitated by AquaPrecipi/isoproanol.
PCR amplification of human stool DNA.
Lane 1: PCR of DNA precipitated by isopropanol
Lane 2: PCR of DNA precipitated by AquaPrecipi/ethanol.
Lane 3: PCR of DNA precipitated by AquaPrecipi/isoproanol.
Hi AquaPlasmid--
Can you clarify, is this for removing inhibitors, post-extraction? Cause I got a lot of failed fecal extractions with inhibitors, but dilution doesn't help because the template copies are already so low/degraded, I would love a way to rescue them...
Thanks
Kim
