6 replies to this topic

[spjgjsm]

Quick question as I have read all sorts of views on this before:

How long can bisufite-treated DNA be stored in -80C freezer before it degrades. Is there any reason why it should degrade quicker than untreated DNA??

Jon

[methylnick]

Jon,

I resuspend my bisulfite treated DNA in fresh autoclaved TE buffer and store at -20C. I have stored such DNA upto 6 months with no detectable loss of DNA and by then I have used up my batch and I have to make another batch.

Hope this helps.

Cheers

Nick

[pcrman]

I agree with Nick. No problem for six months although some suggest no more than one month.

[spjgjsm]

cheers!

jon

[Chris Harris]

Make sure you store in TE, rather than water. Bis treated DNA stored in water goes off quite fast.

[SimonT]

Hi!
But EDTA contained in TE buffer is binding Mg ions (correct me if I'm wrong). So i have to use higher Mg concentration to my PCR?

Regards
Simon

[et2b]

I just store it in the cooler +6 degrees celsius, no problems for more than a year...
had problems with freezing and thawing...thats why....degrades the BS treated DNA even more.
God damned BS DNA....