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Murine blood extraction technique


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4 replies to this topic

#1 daveomahony

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Posted 04 February 2005 - 03:30 AM

ANYONE GOT ANY IDEAS ON HOW TO EXTRACT BLOOD FROM MICE- I FIND CARDIAC PUNCTURE VERY HIT AND MISS?

#2 garita

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Posted 04 February 2005 - 11:05 AM

you should try retro- orbital punction. it's easy to manage under anaesthesia

#3 JunieMoon

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Posted 22 March 2005 - 09:59 PM

If you're doing a terminal proceedure, you may be able to get away with highly anesthetizing, opening the chest and then doing a cardiac puncture...like if going to do a perfusion. If the mice need to be saved for another day, retro-orbital could be done. I've also seen the nicking of a tail vein and collecting a small amount there.

Junie

#4 kristen13711

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Posted 23 March 2005 - 11:49 AM

ANYONE GOT ANY IDEAS ON HOW TO EXTRACT BLOOD FROM MICE- I FIND CARDIAC PUNCTURE VERY HIT AND MISS?

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It depends on how much blood you need. The total blood volume in an animal's body equals approximately 6% of its total body weight. To prevent anemia for nonterminal bleeds the guideline is you can take a volume of 1% of the body weight every two weeks. I have found that orbital sinus bleeding works very well in the mouse model.

#5 jadefalcon

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Posted 24 March 2005 - 01:21 AM

Nicking the tail vein(s) with a sharp instrument like a scalpel gave me enough blood for monitoring antibody-titers, is non-terminal and I personally prefer a cut in the skin to drilling into the mouse's eye (<- dramatization! :) ). The only thing you have to do for successful tail-blooding, is to make sure that the mouse is warmand therefor the peripheral vessels are wide - we use a redlight and put the mice under that 10 minutes before bleeding.

mike
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