Mouse pancreatic primary culture: protocol is needed
Posted 03 February 2005 - 07:31 AM
Does anyone has a reliable protocol to establsih mouse pancreatic primary culture?
Thanks a lot in advance for Your help and time,
Posted 03 February 2005 - 07:55 AM
Posted 03 February 2005 - 08:31 AM
Yes, I am growing it in mouse model now and planning to start the establsihment next week....
Do I have to add any hormonal suppelements (since pancreas is a hormonally active tissue), antibiotics, etc.?
Besides, where do you get 10% calf serum?
Edited by marianna75, 03 February 2005 - 08:33 AM.
Posted 03 February 2005 - 08:40 AM