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Mouse pancreatic primary culture: protocol is needed


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4 replies to this topic

#1 marianna75

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Posted 03 February 2005 - 07:31 AM

Hello!
Does anyone has a reliable protocol to establsih mouse pancreatic primary culture?

Thanks a lot in advance for Your help and time,

Marianna

#2 Jacen

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Posted 03 February 2005 - 07:55 AM

Do you have it growing in mice right now? Best to use DMEM and 10% Fetal Calf Serum as your media and cut up the tumor into small fragments (4-5 mm). Then just incubate and see if the cells adhere. Might need to add 10% mouse serum also if the tumor originated in a mouse.

#3 marianna75

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Posted 03 February 2005 - 08:31 AM

Jancen, thanks..
Yes, I am growing it in mouse model now and planning to start the establsihment next week....
Do I have to add any hormonal suppelements (since pancreas is a hormonally active tissue), antibiotics, etc.?
Besides, where do you get 10% calf serum?

Edited by marianna75, 03 February 2005 - 08:33 AM.


#4 marianna75

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Posted 03 February 2005 - 08:40 AM

...Oh, just now I realized you've been talking about pancreatic tumor tissue whereas I mean normal pancreatic acini..

#5 Jacen

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Posted 03 February 2005 - 09:07 AM

Ooops, my bad, sorry.




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