4 replies to this topic

[marianna75]

Hello!
Does anyone has a reliable protocol to establsih mouse pancreatic primary culture?

Thanks a lot in advance for Your help and time,

Marianna

[Jacen]

Do you have it growing in mice right now?  Best to use DMEM and 10% Fetal Calf Serum as your media and cut up the tumor into small fragments (4-5 mm).  Then just incubate and see if the cells adhere.  Might need to add 10% mouse serum also if the tumor originated in a mouse.

[marianna75]

Jancen, thanks..
Yes, I am growing it in mouse model now and planning to start the establsihment next week....
Do I have to add any hormonal suppelements (since pancreas is a hormonally active tissue), antibiotics, etc.?
Besides, where do you get 10% calf serum?

Edited by marianna75, 03 February 2005 - 08:33 AM.

[marianna75]

...Oh, just now I realized you've been talking about pancreatic tumor tissue whereas I mean normal pancreatic acini..

[Jacen]

Ooops, my bad, sorry.