Hi all,
May anyone tell me the purpose of using different pH, at 8.0 and 7.5, of TE buffers for DNA and RNA disolution, respectively? Can TE at pH 8,0 be used for both DNA and RNA?
Thank you in advance.
0
2 replies to this topic
#2
tfitzwater
-
- Active Members
-
- 96 posts
Enthusiast
2
Neutral
Neutral
Posted 03 February 2005 - 07:37 AM
The use of TE with slightly different pH values is based on early 80's literature that DNases are supposed to be less active at pH 8.0 and RNases less active at pH 7.5.
You can use pH 8.0 for both DNA and RNA.
You can use pH 8.0 for both DNA and RNA.
#3
saibo
-
- Active Members
-
- 6 posts
member
0
Neutral
Neutral
Posted 04 February 2005 - 03:47 AM
Hi tfitzwater,
Thank you for the infos.
saibo
Thank you for the infos.
saibo
