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DNA extraction method for alcohol preserved plant tissues


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#1 Orchidman

Orchidman

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Posted 02 February 2005 - 12:03 PM

:) Hi all
Hope that someone here can help, I have received from a well meaning contact in Trinidad alcohol preserved plant tissues... i should be more clear, he used vodka to preserve the plants I think. Anyway, i have taken 1/2 (>1g) of the leaf tissues and dried them in a vacuum oven, the rest remains in the alcohol. Both are in the freezer to prevent any further degradation, as if that is possible.

I am aware of protocols for DNA extraction from alcohol preserved insect tissues, our bug people here use it fairly reliably, but no one seems to know if there is a protocol for plant tissues that will work; or for that matter if CTAB of some variant might be effective? any suggestions thanks
Erik




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