Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Long term storage of RNA

rna storage long-term rna degradation

  • Please log in to reply
4 replies to this topic

#1 anonymous

anonymous

    Veteran

  • PipPipPipPipPipPipPipPipPipPip
  • 1,890 posts
1
Neutral

Posted 31 July 2001 - 09:00 PM

I am looking for a way to store RNA long term (longer than a few years) without it degrading. Presently we are considering using cryovials to prevent evaporation. Any other suggestions would be great. Thank you.

#2 ragno

ragno

    member

  • Active Members
  • Pip
  • 26 posts
0
Neutral

Posted 26 August 2001 - 09:00 PM

hello,RNA can be stored in microtubes at -80C without degradation but it's important to thaw on ice and centrifuge before use.michel

#3 volans1900

volans1900

    member

  • Members
  • Pip
  • 4 posts
0
Neutral

Posted 12 July 2012 - 09:08 PM

hello,RNA can be stored in microtubes at -80°C without degradation but it's important to thaw on ice and centrifuge before use.michel


May I know why it has to centrifuge before use?

#4 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,788 posts
406
Excellent

Posted 13 July 2012 - 07:19 PM

To make sure that you have all of it in the bottom of the tube...

Incidentally you should also look at the last post date - 2001 - means you probably won't get a response from one of the original posters.

#5 phage434

phage434

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 2,484 posts
251
Excellent

Posted 20 July 2012 - 08:32 AM

I thought typical longer term storage was as a pellet in alcohol at -80.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.