product size for real-time PCR primer
Posted 01 February 2005 - 06:37 PM
I have primers that give product size of 420bp. Can I use them for real-time PCR?
I usually hear that real-time pcr primes should give a product size of 75-150. What is the reason for that?
I'll appreciate any replies.
Thank you very much.
Posted 02 February 2005 - 06:59 AM
Posted 02 February 2005 - 08:36 PM
According to the link from littlecell: