Posted 27 August 2001 - 09:00 PM
Posted 02 September 2001 - 09:00 PM
The sucrose gradient it is not a good method for protein separation (especially with a small MW). You will lost a lot of time for installation and irreproducible resulst. As I think, the best method for solving your problem is the ion-exange or gel-permeation chromatografy. These methods are highly reproduceble and simple for use.Good luck,Oleg Klychnikov, PhD.