What is the best transfection method for CaCo2 cell lines? I have tried Fugene6 and have not had much luck. I have heard that Gene Juice by Novagene will work. I am also looking into Jet PEI from Obiogene. Any other suggestions?
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#1
dadle
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Posted 29 January 2005 - 11:24 AM
What is the best transfection method for CaCo2 cell lines? I have tried Fugene6 and have not had much luck. I have heard that Gene Juice by Novagene will work. I am also looking into Jet PEI from Obiogene. Any other suggestions?
#2
BryanW
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Posted 30 January 2005 - 01:50 PM
The most effective liposome-based transfection reagents are GenCarrier, Lipofectamine2000(CD) followed by fugene-6 and genePORTER. In our hands, GenCarrier tops the list in low cytotoxicity, high transfection efficiency, much lower price and broad cell types - primary or established, adherant or suspension, serum or no serum.
#3
expresson_help
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Posted 03 February 2005 - 08:25 AM
You might also consider NeoPhectin, which is good for difficult to transfect cell types.
