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I have been using ammonium chloride buffer. Typically, this method requries that I repeat the procedure on my sample several times before I get any lysis at all.
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red blood cell lysis
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blossom 
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Posted 28 January 2005 - 01:09 PM
Does anyone know the BEST way to lyse red blood cells from mouse peripheral blood (preserved in 0.05 mM EDTA solution) for FACS analysis.
I have been using ammonium chloride buffer. Typically, this method requries that I repeat the procedure on my sample several times before I get any lysis at all.
I have been using ammonium chloride buffer. Typically, this method requries that I repeat the procedure on my sample several times before I get any lysis at all.
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