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Competent Bascillus Cells - Molecular Biology - BioForum

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Competent Bascillus Cells


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#1 anonymous

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Posted 25 September 2001 - 09:00 PM

Add powder glycine to 1.0 M when bacillus culture OD reached 0.7 at 650nm. Let grow for another 2 hours. The cells are highly permeable right now to DNA. If this does not work, zap the #### things using an electroporator.

Good luck

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#2 anonymous

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Posted 25 September 2001 - 09:00 PM

Hello,I am looking for a protocol to prepare competent Bascillus subtilis cells.Any help would be greatly appreciated.Emre




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