Which vector for cloning big PCR fragments?
Posted 28 January 2005 - 06:45 AM
Posted 28 January 2005 - 12:13 PM
You may have to use BACs for that size insert, and you have to cut it with a restriction enzyme and ligate it into the MCS of the BAC.
Posted 30 January 2005 - 03:47 AM
God helps them who help themselves!
Posted 31 January 2005 - 04:06 AM
i used LATaq polymerase TAKARA inc.
they say you can amplify 35 kb. i arrived to 19kb. part of a natural plasmid in V. cholerae!thanks a lot