I have a problem with co-expression in e. coli.
I co-transformed 2 vectors carrying 2 different proteins into e. coli
I used pRSET and pMON (p15A ori, kan resistant)
i tried 2 host strains: BL21(DE3) and BL21(DE3)pLysS
protein in pRSET was expressed well in pLysS but one in pMON wasn't;
and protein in pMON was expressed well in BL21(DE3) but one in pRSET wasn't.
both proteins were not expressed well in both strains.
(couldn't see expression on SDS-PAGE)
how can i improve the expression (w/o changing vectors)?
would it help if i change hosts strains?
which strains can i use...?
I really appreciate if someone can help.
Edited by ad1980, 26 January 2005 - 09:44 PM.