Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

phospho-specific antibody


  • Please log in to reply
1 reply to this topic

#1 patirl

patirl

    member

  • Active Members
  • Pip
  • 10 posts
0
Neutral

Posted 26 January 2005 - 10:25 AM

Hi again

Another problem. I'm using a phospho-specific antibody and I'm having problems. The antibody recognises another protein at the same size as the protein of interest. As a control I have a 18myc-tagged version of the protein. the phospho-antibody recognises the myc-tagged protein but also a protein at the same size as the wild type. I don't think that it is my protein of interest as a non-phospho antibody only recognises the myc-tagged version of the protein and not this mysterious X protein.

Although my problem may not sound very clear, does anyone have any ideas how I can remove this background band?

#2 Oleksii

Oleksii

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 31 posts
0
Neutral

Posted 30 January 2005 - 05:21 PM

Try 2D gel. In theory, it should resolve any proteins.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.