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problems with yeast protein extract


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#1 patirl

patirl

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Posted 26 January 2005 - 10:18 AM

Hi all

I'm having troubles making yeast protein extracts. When I use the TCA method my samples run as normal but I have problems with quantification of my samples. Any ideas about that?

However, whenever I try to use a Tris-based lysis buffer followed by bead beating the samples always run strangely. The bands are sometimes triangular in shape. What could this mean? I am really confused.

Can anyone help?

Thanks

Pat




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