I'm trying to generate heterokaryons from 2 cell lines, namely U937 (monocytes) and Hela cells. I'v tried different protocols and used commercial PEG from Sigma but I got only 3% of fused cells and which don't survive after FACS sorting. Problem is : one cell line is adherent and the other is not. Has anybody had the same problem and who could help me out on this ?
No replies to this topic