I'm wondering if using BGS to grow a cell line developed using FBS can change cell biology.
I am starting to have negative experimental results after several positives in the same assay. The serum change is one of a few variables I am trying to eliminate, to see what is the problem.
In my case, now I have low or no Erk1/2 activation were I had high or some (respectivelly).
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BGS vs. FBS
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