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The role of NP-40 vs Triton X-100


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#1 Cindyflying

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Posted 24 January 2005 - 09:59 AM

I found a lot of lysis buffer are different in terms of non-denaturing reagents, such as NP-40 AND Triton X-100. But does both of them have different roles? I am wondering...

#2 badcell

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Posted 27 January 2005 - 09:02 AM

According to the Sigma product info sheet:

Triton X-100tm has a structure very similar to those of Igepal CA-630 and of Nonidet P-40 (no longer commercially available), and the names are sometimes reported as synonyms. However, Triton X-100 is slightly more hydrophilic than Igepal CA-630; these two detergents are NOT considered to be functionally interchangeable in most applications.

In my hands, NP40 is more stringent than TX100 when used at the same percentages.
Science is a wonderful thing if one does not have to earn one's living at it
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