3 replies to this topic

[shadab]

I want to extract DNA from moderately halophilic gram negative bacteria and do PCR. Does anyone an experience in this subject? would you tell me which protocol you used?

Thanks

[leahf]

what do you mean by "moderately halophilic"? is it found in seawater or extremly saline environment?

[shadab]

It was found in salty soil  and needs at least 0.5 to 1 g/litter NaCl.

[leahf]

that's not so halophilic-  try the normal protocols for bacterial DNA isolation - the classic phenol/chlorophorm extraction or commercial kits (sigma, promega, etc.) - we have used all of those for extracting DNA from marine bacteria (grown with 2 - 4 % NaCl) with no problems.