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How to extract DNA from halophilic bacteria?


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3 replies to this topic

#1 shadab

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Posted 23 January 2005 - 11:10 AM

I want to extract DNA from moderately halophilic gram negative bacteria and do PCR. Does anyone an experience in this subject? would you tell me which protocol you used?

Thanks

#2 leahf

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Posted 25 January 2005 - 09:24 AM

what do you mean by "moderately halophilic"? is it found in seawater or extremly saline environment?

#3 shadab

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Posted 04 February 2005 - 09:50 AM

It was found in salty soil and needs at least 0.5 to 1 g/litter NaCl.

#4 leahf

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Posted 06 February 2005 - 04:04 AM

that's not so halophilic- try the normal protocols for bacterial DNA isolation - the classic phenol/chlorophorm extraction or commercial kits (sigma, promega, etc.) - we have used all of those for extracting DNA from marine bacteria (grown with 2 - 4 % NaCl) with no problems.




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