pkmishra, on Jan 22 2005, 02:50 AM, said:
I
m a fello of molecular biology. if there is scientist who want to suggest any topic or line of work which can contribute the knowledge abuout cancer and DNA methylation + early detection using simplest technique please guide me.
Maybe start your search into the field of epigenetics here: you do know how to use Pubmed do you?
Plasma DNA methylation profiling might be promising in the future, no cutting into people; which is always a nice thingy to avoid
There are also plenty of reviews about using DNA methylation for cancer detection etc etc. Just google or pubmed and you will find it.... Are you sure that you are in molecular biology? You ask M.D. like questions...(sorry)
Methylation profile of circulating plasma DNA in patients with pancreatic cancer.Melnikov AA, Scholtens D, Talamonti MS, Bentrem DJ, Levenson VV.
Robert H Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA.
BACKGROUND AND OBJECTIVES: Detection of pancreatic cancer by blood-based test may improve outcomes. We sought to establish the feasibility of a blood-based detection of pancreatic cancer through multiplexed array-mediated analysis of DNA methylation. METHODS: Methylation was assessed in each plasma sample using a panel of 56 frequently methylated genes. Methylation profiles in patients with ductal cell adenocarcinoma of the pancreas (n = 30) and healthy gender and age-matched controls (n = 30) were compared. Methylation was determined as described previously; a composite biomarker was developed for classification of cancer and normal samples. Sensitivity and specificity of the biomarker were estimated using 25 rounds of fivefold cross-validation. RESULTS: Five promoters were consistently selected for the classifier during cross-validation and comprised the final composite biomarker Five-fold cross-validation results indicate 76% sensitivity and 59% specificity of the biomarker, which included promoters of CCND2, SOCS1, THBS1, PLAU, and VHL. CONCLUSION: Differential methylation profiling of plasma DNA can detect ductal adenocarcinoma of the pancreas with significant accuracy and should be explored further. While additional improvement of biomarkers is necessary, the blood-based biomarker may be already useful as a first-line detection tool.
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