RNA Extraction using Trizol
Posted 21 January 2005 - 06:33 AM
Posted 21 January 2005 - 08:24 AM
Posted 21 January 2005 - 10:35 AM
Posted 21 January 2005 - 12:18 PM
badcell, on Jan 21 2005, 11:35 AM, said:
Posted 21 January 2005 - 03:17 PM
You can use 20 ug per sample. That amount will allow you to precipitate pg-amount of RNA from a volume of 1 ml.
Posted 24 January 2005 - 07:32 AM
Yeah, you don't need to add glycogen if your sample is big enough, but I found that glycogen is necessary when working with small samples of tissue or cells. It increases a lot the quantity of RNA you can extract. I usually work with as few as 15000 cells and I got enough RNA to measure OD, run in an gel and do RT. I would certainly add glycogen for a sample of 1 mg tissue.