cloning in pQE30
Posted 21 January 2005 - 06:21 AM
I am not able to clone my pcr product in pQE 30 for expression. On the gel and in transformation results, it looks like digestion and dephosphorylation is working.
When I do the colony PCR for the presence of insert, I get right sized fragment. But when I do PCR for vector specific and insert specific primers, I get wrong bands. I also got my clones sequenced, but result shows no inset sequences.
could someone please help me ???
Posted 21 January 2005 - 07:30 AM
Posted 21 January 2005 - 08:29 AM