Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

protein solubility in TCA

  • Please log in to reply
1 reply to this topic

#1 ecsl



  • Members
  • Pip
  • 1 posts

Posted 20 January 2005 - 02:35 PM

I am working with salivary proteins and I saw, several times, protocols in which the proline rich proteins are isolated by mixing the saliva sample with an equal volume of TCA 10%. I do not understand the basis for that. Can someone explain me why this type of proteins are soluble in TCA? What makes them different from the other proteins?
Thank you

#2 thefallguy



  • Active Members
  • Pip
  • 27 posts

Posted 20 January 2005 - 05:31 PM

TCA is used for two purposes- to remove nucleic acids by precipitation and to make the otherwise insoluble proline rich proteins more soluble-does not always work because every protein is different. You don't have to use this method. Nucleic acids can be removed by adding trace amounts of RNaseI and DNaseIII- while proline rich proteins go into solution at 0.75M NaCl strength in any pH 8.0 buffer.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.