Hi
I am working with salivary proteins and I saw, several times, protocols in which the proline rich proteins are isolated by mixing the saliva sample with an equal volume of TCA 10%. I do not understand the basis for that. Can someone explain me why this type of proteins are soluble in TCA? What makes them different from the other proteins?
Thank you
ecsl
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thefallguy
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Posted 20 January 2005 - 05:31 PM
TCA is used for two purposes- to remove nucleic acids by precipitation and to make the otherwise insoluble proline rich proteins more soluble-does not always work because every protein is different. You don't have to use this method. Nucleic acids can be removed by adding trace amounts of RNaseI and DNaseIII- while proline rich proteins go into solution at 0.75M NaCl strength in any pH 8.0 buffer.
