Hi,
I was wondering if any one has tried aminoallyl labeling before and what your opinions are in terms of qulaity of the results, the incorporation of the cy3/cy5 dyes and whether or not there is any dye bias issues to deal with when analyzing the microarray data?
Thank You.
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Posted 02 February 2005 - 07:22 PM
in my experience amino-allyl labelling works well, although Cy3 ALWAYS works better than Cy5.
there is definately a dye bias for Cy3 in preference to Cy5. but as long as you do reverse labelling controls, you should be fine.
there is definately a dye bias for Cy3 in preference to Cy5. but as long as you do reverse labelling controls, you should be fine.
