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what's the proper amout of 16SrDNA should I transfer to DGGE

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#1 bioforumdh



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Posted 19 January 2005 - 09:37 PM

After PCR of 16SrDNA,I subject 16SrDNA to DGGE,but I don't know what's
the proper amount of 16SrDNA should I transfer to DGGE,I mean if I add
more than the proper amount,It would be wasteful;if I add less than the
proper amount,some bands couldn't be detected by DGGE.
Could you please give me some suggestion about it?

Edited by bioforumdh, 19 January 2005 - 09:39 PM.

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