i have tried three different kits for mini-prep plasmid DNA purification. but i could not get rid of the chrosomal DNA contamination though i strictly followed the manual of each producer.
could anyone give me any advice? thanks in advance.
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Plasmid DNA Purification
Started by littlecell, Jan 19 2005 08:18 AM
1 reply to this topic
#1
littlecell
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Posted 19 January 2005 - 08:18 AM
hello, everybody.
i have tried three different kits for mini-prep plasmid DNA purification. but i could not get rid of the chrosomal DNA contamination though i strictly followed the manual of each producer.
could anyone give me any advice? thanks in advance.
i have tried three different kits for mini-prep plasmid DNA purification. but i could not get rid of the chrosomal DNA contamination though i strictly followed the manual of each producer.
could anyone give me any advice? thanks in advance.
#2
mybbff
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Posted 19 January 2005 - 07:08 PM
Try to reduce the lysis time in step2. And do not extend 5min.
Edited by mybbff, 19 January 2005 - 07:09 PM.
