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cloning big fragments


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#1 danielaceccarelli

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Posted 19 January 2005 - 08:11 AM

hi!
i'd like to know if someone of you know a good vector in which i can clone big fragment (from a digested plasmid). with big i mean 15-20 kb!
no lambda please!
thanks
d :)

#2 Sequencer

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Posted 17 March 2005 - 02:35 AM

i used a fosmid vector...copy control fosmid library production kit from epicentre... i tried using bac but way too complicated for me...




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