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Protease inhibitor--Aprotinin activity


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#1 Cindyflying

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Posted 18 January 2005 - 08:28 PM

I want to prepare a lysis buffer, which contains several proteinase inhibitor, among those is Aprotinin. I bought it from Fisher Company. Its total volume is 10ml. The instruction said the activity is 10,000KIU/ml, but I want to first make a working solution 1 mg/ml, so I don't know what is its concentration. I searched and found there is a conversion factor: 1 KIU/L= 0.83 ng/ml. Is it right? Please give me the easiest way to make this solution. Thanks!

#2 Cindyflying

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Posted 23 January 2005 - 05:24 PM

Does anybody know about this? Please reply it soon! I'll appreciate a lot!




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